Activity of granulomatosis with polyangiitis and its correlation with mTOR phosphoproteomics in neutrophils.

ABSTRACT:

Introduction

Granulomatosis with polyangiitis (GPA) is a small vessel vasculitis with a complex pathomechanism. Organ damage in GPA is also mediated by extracellular trap formation (NETosis). We analyzed the functional status of phosphoproteins modulating NETosis in neutrophils by the mammalian target of rapamycin (mTOR) pathway in GPA along with NETosis biomarkers.

Methods

Phosphoproteins levels measured in isolated neutrophils from 42 patients with GPA (exacerbation n=21; remission n=21) and 21 healthy controls were compared to serum biomarkers of the disease.

Results

Neutrophils in active disease manifested lowered levels of phosphorylated mTOR^(Ser2448), PTEN^(Ser380) and ULK1^(Ser555), whereas phosphorylated GSK-3α/β^(Ser21/Ser9)

SUBMITTER: Surmiak M 

PROVIDER: S-EPMC10500300 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature