Strategies to identify and suppress crosstalk signals in double electron-electron resonance (DEER) experiments with gadolinium^III and nitroxide spin-labeled compounds.

ABSTRACT: Double electron-electron resonance (DEER) spectroscopy applied to orthogonally spin-labeled biomolecular complexes simplifies the assignment of intra- and intermolecular distances, thereby increasing the information content per sample. In fact, various spin labels can be addressed independently in DEER experiments due to spectroscopically nonoverlapping central transitions, distinct relaxation times, and/or transition moments; hence, they are referred to as spectroscopically orthogonal. Molecular complexes which are, for example, orthogonally spin-labeled with nitroxide (NO) and gadolinium (Gd) labels give access to three distinct DEER channels that are optimized to selectively probe NO-NO, NO-Gd, and Gd-Gd distances. Nevertheless, it has been previously recognized that crosstalk signals b

SUBMITTER: Teucher M 

PROVIDER: S-EPMC10500692 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature