Identification of exosomal microRNA panel as diagnostic and prognostic biomarker for small cell lung cancer.

ABSTRACT:

Background

Small cell lung cancer (SCLC) has an exceptionally poor prognosis; as most of the cases are initially diagnosed as extensive disease with hematogenous metastasis. Therefore, the early diagnosis of SCLC is very important and may improve its prognosis.

Methods

To investigate the feasibility of early diagnosis of SCLC, we examined exosomal microRNAs (miRNAs) present in serum obtained from patients with SCLC. First, exosomes were isolated in serum from patients with SCLC and healthy individuals and were characterized using particle size and protein markers. Additionally, miRNA array was performed to define SCLC-specific exosomal miRNAs. Second, the obtained miRNAs were further validated employing a large cohort. Finally, the ability to diagnose SCLC was estimated by a

SUBMITTER: Kim DH 

PROVIDER: S-EPMC10500735 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature