Dataset Information

Loss of Dip2b leads to abnormal neural differentiation from mESCs.

ABSTRACT:

Background

Disco-interacting protein 2 homolog B is a member of the Dip2 family encoded by the Dip2b gene. Dip2b is widely expressed in neuro-related tissues and is essential in axonal outgrowth during embryogenesis.

Methods

Dip2b knockout mouse embryonic stem cell line was established by CRISPR/Cas9 gene-editing technology. The commercial kits were utilized to detect cell cycle and growth rate. Flow cytometry, qRT-PCR, immunofluorescence, and RNA-seq were employed for phenotype and molecular mechanism assessment.

Results

Our results suggested that Dip2b is dispensable for the pluripotency maintenance of mESCs. Dip2b knockout could not alter the cell cycle and proliferation of mECSs, or the ability to differentiate into three germ layers in vitro. Furthermore, genes associated with axon guidance, channel activity, and synaptic membrane were significantly downregulated during neural differentiation upon Dip2b knockout.

Conclusions

Our results suggest that Dip2b plays an important role in neural differentiation, which will provide a valuable model for studying the exact mechanisms of Dip2b during neural differentiation.

SUBMITTER: Yao M

PROVIDER: S-EPMC10500737 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Publications

Loss of Dip2b leads to abnormal neural differentiation from mESCs.

Yao Mingze M Pan Yuanqing Y Ren Tinglin T Yang Caiting C Lei Yu Y Xing Xiaoyu X Zhang Lei L Cui Xiaogang X Zheng Yaowu Y Xing Li L Wu Changxin C

Stem cell research & therapy 20230913 1

<h4>Background</h4>Disco-interacting protein 2 homolog B is a member of the Dip2 family encoded by the Dip2b gene. Dip2b is widely expressed in neuro-related tissues and is essential in axonal outgrowth during embryogenesis.<h4>Methods</h4>Dip2b knockout mouse embryonic stem cell line was established by CRISPR/Cas9 gene-editing technology. The commercial kits were utilized to detect cell cycle and growth rate. Flow cytometry, qRT-PCR, immunofluorescence, and RNA-seq were employed for phenotype a ...[more]

PMID: 37705068

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Dataset Information

Loss of Dip2b leads to abnormal neural differentiation from mESCs.

Background

Methods

Results

Conclusions

Publications

Loss of Dip2b leads to abnormal neural differentiation from mESCs.

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