Isolation and genetic characterization of MERS-CoV from dromedary camels in the United Arab Emirates.

ABSTRACT:

Background

The study of coronaviruses has grown significantly in recent years.Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in various cell types, and quick development has been made of assays for its growth and quantification. However, only a few viral isolates are now available for investigation with full characterization. The current study aimed to isolate MERS-CoV from nasal swabs of dromedary camels and molecularly analyze the virus in order to detect strain-specific mutations and ascertain lineage classification.

Methods

We isolated the virus in Vero cells and adapted it for in vitro cultivation. The isolates were subjected to complete genome sequencing using next-generation sequencing followed by phylogenetic, mutation, and recombination an

SUBMITTER: Khalafalla AI 

PROVIDER: S-EPMC10500840 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature