Biodistribution of mesenchymal stromal cell-derived extracellular vesicles administered during acute lung injury.

ABSTRACT:

Background

Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) are a promising cell-free therapy for acute lung injury (ALI). To date, no studies have investigated their biodistribution in ALI or discerned the timing of administration for maximal lung targeting, which are crucial considerations for clinical translation. Our study aimed to characterize a mouse model of ALI and establish the distribution kinetics and optimal timing of MSC-EV delivery during lung injury.

Methods

MSC-EVs were isolated by ultracentrifugation alone (U/C) or tangential flow filtration with ultracentrifugation (TFF-U/C) and characterized by nanoparticle tracking analysis and western blot. A lipopolysaccharide (LPS)-induced mouse model of ALI was established to study the inflammatory re

SUBMITTER: Tieu A 

PROVIDER: S-EPMC10500845 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature