Controlled Protein Activities with Viral Proteases, Antiviral Peptides, and Antiviral Drugs.

ABSTRACT: Chemical control of protein activity is a powerful tool for scientific study, synthetic biology, and cell therapy; however, for broad use, effective chemical inducer systems must minimally crosstalk with endogenous processes and exhibit desirable drug delivery properties. Accordingly, the drug-controllable proteolytic activity of hepatitis C cis-protease NS3 and its associated antiviral drugs have been used to regulate protein activity and gene modulation. These tools advantageously exploit non-eukaryotic and non-prokaryotic proteins and clinically approved inhibitors. Here, we expand the toolkit by utilizing catalytically inactive NS3 protease as a high affinity binder to genetically encoded, antiviral peptides. Through our approach, we create NS3-peptide complexes that can be disp

SUBMITTER: Tague EP 

PROVIDER: S-EPMC10501127 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature