The effects of coagulation factors and their inhibitors on proliferation and migration in colorectal cancer.

ABSTRACT:

Background/aim

Clotting factors promote cancer development. We investigated if coagulation proteins promote proliferation and migration in colorectal cancer (CRC) cell lines and whether their direct inhibitors can attenuate these effects.

Materials and methods

DLD-1 and SW620 cells were treated with tissue factor (0, 50, 100 and 500 pg/mL ± 10 μg/mL 10H10 [anti-tissue factor antibody]), thrombin (0.0, 0.1, 1.0 and 10.0 U/mL ± 0.5 μM dabigatran [thrombin inhibitor]) and Factor Xa, FXa (0.0, 0.1, 1.0 and 10.0 U/mL ± 100 ng/mL rivaroxaban [FXa inhibitor]) and their effects on proliferation and migration were quantified using the PrestoBlue® and transwell migration assays, respectively.

Results

Thrombin increased proliferation from 48 h treatment compared to its control (

SUBMITTER: Rees PA 

PROVIDER: S-EPMC10501231 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature