Robust Accurate Identification and Biomass Estimates of Microorganisms via Tandem Mass Spectrometry.

ABSTRACT: Rapid and accurate identification of microorganisms and estimation of their biomasses are of extreme importance to public health. Mass spectrometry has become an important technique for these purposes. Previously we published a workflow named Microorganism Classification and Identification (MiCId v.12.26.2017) that was shown to perform no worse than other workflows. This manuscript presents MiCId v.12.13.2018 that, in comparison with the earlier version v.12.26.2017, allows for biomass estimates, provides more accurate microorganism identifications (better controls the number of false positives), and is robust against database size increase. This significant advance is made possible by several new ingredients introduced: first, we apply a modified expectation-maximizat

SUBMITTER: Alves G 

PROVIDER: S-EPMC10501333 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature