Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa.

ABSTRACT: Fungal cells are quite unique among life in their organization and structure, and yet implementation of many tools recently developed for fluorescence imaging in animal systems and yeast has been slow in filamentous fungi. Here we present analysis of properties of fluorescent proteins in Neurospora crassa as well as describing genetic tools for the expression of these proteins that may be useful beyond cell biology applications. The brightness and photostability of ten different fluorescent protein tags were compared in a well-controlled system; six different promoters are described for the assessment of the fluorescent proteins and varying levels of expression, as well as a customizable bidirectional promoter system. We present an array of fluorescent proteins suitable for use across the

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10501358 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature