Large-Scale and Site-Specific Mapping of the Murine Brain O-Glycoproteome with IMPa.

ABSTRACT: Altered protein glycosylation is typically associated with cognitive defects and other phenotypes, but there is a lack of knowledge about the brain glycoproteome. Here, we used the newly available O-glycoprotease IMPa from Pseudomonas aeruginosa for comprehensive O-glycoproteomic analyses of the mouse brain. In this approach, total tryptic glycopeptides were prepared, extracted, purified, and conjugated to a solid support before an enzymatic cleavage by IMPa. O-glycopeptides were analyzed by electron-transfer/higher-energy collision dissociation (EThcD), which permits site-specific and global analysis of all types of O-glycans. We developed two complementary approaches for the analysis of the total O-glycoproteome using HEK293 cells and derivatives

SUBMITTER: Suttapitugsakul S 

PROVIDER: S-EPMC10501376 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature