Factor VIII antibody immune complexes modulate the humoral response to factor VIII in an epitope-dependent manner.

ABSTRACT:

Introduction

Soluble antigens complexed with immunoglobulin G (IgG) antibodies can induce robust adaptive immune responses in vitro and in animal models of disease. Factor VIII immune complexes (FVIII-ICs) have been detected in individuals with hemophilia A and severe von Willebrand disease following FVIII infusions. Yet, it is unclear if and how FVIII-ICs affect antibody development over time.

Methods

In this study, we analyzed internalization of FVIII complexed with epitope-mapped FVIII-specific IgG monoclonal antibodies (MAbs) by murine bone marrow-derived dendritic cells (BMDCs) in vitro and antibody development in hemophilia A (FVIII^-/-) mice injected with FVIII-IC over time.

Results

FVIII complexed with 2-116 (A1 domain MAb), 2-113 (A3 d

SUBMITTER: Batsuli G 

PROVIDER: S-EPMC10501482 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature