Biosensor-integrated transposon mutagenesis reveals <i>rv0158</i> as a coordinator of redox homeostasis in <i>Mycobacterium tuberculosis</i>.

ABSTRACT: Mycobacterium tuberculosis (Mtb) is evolutionarily equipped to resist exogenous reactive oxygen species (ROS) but shows vulnerability to an increase in endogenous ROS (eROS). Since eROS is an unavoidable consequence of aerobic metabolism, understanding how Mtb manages eROS levels is essential yet needs to be characterized. By combining the Mrx1-roGFP2 redox biosensor with transposon mutagenesis, we identified 368 genes (redoxosome) responsible for maintaining homeostatic levels of eROS in Mtb. Integrating redoxosome with a global network of transcriptional regulators revealed a hypothetical protein (Rv0158) as a critical node managing eROS in Mtb. Disruption of rv0158 (rv0158 KO) impaired growth, redox balance, respiration, and metabolism of

SUBMITTER: Shee S 

PROVIDER: S-EPMC10501769 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature