Profiling tear film enzymes reveals major metabolic pathways involved in the homeostasis of the ocular surface.

ABSTRACT: The ocular surface (OS) enzymes are of great interest due to their potential for novel ocular drug development. We aimed first to profile and classify the enzymes of the OS to describe major biological processes and pathways that are involved in the maintenance of homeostasis. Second, we aimed to compare the enzymatic profiles between the two most common tear collection methods, capillary tubes (CT) and Schirmer strips (ScS). A comprehensive tear proteomic dataset was generated by pooling all enzymes identified from nine tear proteomic analyses of healthy subjects using mass spectrometry. In these studies, tear fluid was collected using CT (n = 4), ScS (n = 4) or both collection methods (n = 1). Classification and functional analysis of the enzymes was performed using a combination of bioi

SUBMITTER: Akkurt Arslan M 

PROVIDER: S-EPMC10502076 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature