Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation.

ABSTRACT: Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we constructed at single-cell resolution, a spatio-temporal transcriptome of cell populations in the germ-layers of gastrula-stage mouse embryos. This molecular atlas enables the inference of molecular network activity underpinning the specification and differentiation of the germ-layer tissue lineages. Heterogeneity analysis of cellular composition at defined positions in the epiblast revealed progressive diversification of cell types. The single-cell transcriptome revealed an enhanced BMP signal

SUBMITTER: Wang R 

PROVIDER: S-EPMC10502153 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature