Cytosine base editors optimized for genome editing in potato protoplasts.

ABSTRACT: In this study, we generated and compared three cytidine base editors (CBEs) tailor-made for potato (Solanum tuberosum), which conferred up to 43% C-to-T conversion of all alleles in the protoplast pool. Earlier, gene-edited potato plants were successfully generated by polyethylene glycol-mediated CRISPR/Cas9 transformation of protoplasts followed by explant regeneration. In one study, a 3-4-fold increase in editing efficiency was obtained by replacing the standard Arabidopsis thaliana AtU6-1 promotor with endogenous potato StU6 promotors driving the expression of the gRNA. Here, we used this optimized construct (SpCas9/StU6-1::gRNA1, target gRNA sequence GGTC₄C₅TTGGAGC₁₂AAAAC₁₇TGG) for the generation of CBEs tai

SUBMITTER: Westberg I 

PROVIDER: S-EPMC10502308 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature