Leveraging polymer modeling to reconstruct chromatin connectivity from live images.

ABSTRACT: Chromosomal dynamics plays a central role in a number of critical biological processes, such as transcriptional regulation, genetic recombination, and DNA replication. However, visualization of chromatin is generally limited to live imaging of a few fluorescently labeled chromosomal loci or high-resolution reconstruction of multiple loci from a single time frame. To aid in mapping the underlying chromosomal structure based on parsimonious experimental measurements, we present an exact analytical expression for the evolution of the polymer configuration based on a flexible-polymer model, and we propose an algorithm that tracks the polymer configuration from live images of chromatin marked with several fluorescent marks. Our theory identifies the resolution of microscopy needed to achieve hi

SUBMITTER: Dutta S 

PROVIDER: S-EPMC10502477 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature