Latent bloodstain detection using a selective turn-on NIR fluorescence dye responsive to serum albumin.

ABSTRACT: Bloodstain detection can provide crucial information and evidence at a crime scene; however, the ability to selectively detect bloodstains in a non-destructive manner with high sensitivity and low background is limited. This study reports a fluorescent dye (sulfonate indolizine squaraine, SO₃SQ) for bloodstain visualization under near-infrared (NIR) irradiation. While the dye itself is minimally fluorescent in aqueous solution, it exhibits a "turn-on" mechanism upon binding with human serum albumin (HSA) as the fluorescence intensity increases over 160 times with strong absorption and emission at 693 nm and 758 nm, respectively. Bloodstains can be visualized on a surface even after being diluted 1000 times, and washed latent bloodstains can be detected with high sensitivity. Fur

SUBMITTER: Qu J 

PROVIDER: S-EPMC10502805 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature