Deep RNA-seq of male and female murine sensory neuron subtypes after nerve injury.

ABSTRACT:

Abstract

Dorsal root ganglia (DRG) neurons have been well described for their role in driving both acute and chronic pain. Although nerve injury is known to cause transcriptional dysregulation, how this differs across neuronal subtypes and the impact of sex is unclear. Here, we study the deep transcriptional profiles of multiple murine DRG populations in early and late pain states while considering sex. We have exploited currently available transgenics to label numerous subpopulations for fluorescent-activated cell sorting and subsequent transcriptomic analysis. Using bulk tissue samples, we are able to circumvent the issues of low transcript coverage and drop-outs seen with single-cell data sets. This increases our power to detect novel and even subtle changes in gene expression w

SUBMITTER: Barry AM 

PROVIDER: S-EPMC10502896 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature