Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome.

ABSTRACT:

Background

In the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct an efficient exocrine expression system for the different heterologous proteins. Yarrowia lipolytica possesses unique advantages in nascent protein transport and glycosylation modification, so it can serve as a potential protein expression platform. Although the Po1 series derived from W29 is often used for the expression of the various heterologous proteins, the ability of W29 to secrete proteins has not been verified and the Po1 series has been found to be not convenient for further gene editing.

Results

A total of 246 Y. lipolytica strains were evaluated for their secretory capacity through performing high-throughput screeni

SUBMITTER: Yu S 

PROVIDER: S-EPMC10503192 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature