Smartphone-read phage lateral flow assay for point-of-care detection of infection.

ABSTRACT: The COVID-19 pandemic has highlighted the urgent need for sensitive, affordable, and widely accessible testing at the point of care. Here we demonstrate a new, universal LFA platform technology using M13 phage conjugated with antibodies and HRP enzymes that offers high analytical sensitivity and excellent performance in a complex clinical matrix. We also report its complete integration into a sensitive chemiluminescence-based smartphone-readable lateral flow assay for the detection of SARS-CoV-2 nucleoprotein. We screened 84 anti-nucleoprotein monoclonal antibody pairs in phage LFA and identified an antibody pair that gave an LoD of 25 pg mL^-1 nucleoprotein in nasal swab extract using a FluorChem gel documentation system and 100 pg mL^-1 when the test was imaged and an

SUBMITTER: Chabi M 

PROVIDER: S-EPMC10503656 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature