Cell-matrix interactions control biliary organoid polarity, architecture, and differentiation.

ABSTRACT:

Background and aims

Cholangiopathies are an important cause of morbidity and mortality. Their pathogenesis and treatment remain unclear in part because of the lack of disease models relevant to humans. Three-dimensional biliary organoids hold great promise; however, the inaccessibility of their apical pole and the presence of extracellular matrix (ECM) limits their application. We hypothesized that signals coming from the extracellular matrix regulate organoids' 3-dimensional architecture and could be manipulated to generate novel organotypic culture systems.

Approach and results

Biliary organoids were generated from human livers and grown embedded into Culturex Basement Membrane Extract as spheroids around an internal lumen (EMB). When removed from the EMC, biliary organoid

SUBMITTER: Fiorotto R 

PROVIDER: S-EPMC10503667 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature