Real time ex vivo chemosensitivity assay for pancreatic adenocarcinoma.

ABSTRACT:

Background

Patient-derived organoids (PDOs) and xenografts (PDXs) have been extensively studied for drug-screening. However, their usage is limited due to lengthy establishment time, high engraftment failure rates and different tumor microenvironment from original tumors. To overcome the limitations, we developed real time-live tissue sensitivity assay (RT-LTSA) using fresh tumor samples.

Methods

Tissue slices from resected pancreatic cancer samples were placed in 96-well plates, and the slices were treated with chemotherapeutic agents. The correlation between the chemo-sensitivity of tissue slices and each patient's clinical outcome was analyzed.

Results

The viability and tumor microenvironment of the tissue slices are well-preserved over 5 days. The drug sensitivity

SUBMITTER: Kim DW 

PROVIDER: S-EPMC10503742 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature