Ion exchange chromatography as a simple and scalable method to isolate biologically active small extracellular vesicles from conditioned media.

ABSTRACT: In the last few years, extracellular vesicles (EVs) have become of great interest due to their potential as biomarkers, drug delivery systems, and, in particular, therapeutic agents. However, there is no consensus on which is the best way to isolate these EVs. The choice of the isolation method depends on the starting material (i.e., conditioned culture media, urine, serum, etc.) and their downstream applications. Even though there are numerous methods to isolate EVs, few are compatible with clinical applications as they are not scalable. In the present work, we set up a protocol to isolate EVs from conditioned media by ion exchange chromatography, a simple, fast, and scalable method, suitable for clinical production. We performed the isolation using an anion exchange resin (Q sepharose) a

SUBMITTER: Malvicini R 

PROVIDER: S-EPMC10503763 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature