A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions.

ABSTRACT: The ability of endothelial cells to respond to blood flow is fundamental for the correct formation and maintenance of a functional and hierarchically organized vascular network. Defective flow responses, in particular related to high flow conditions, have been associated with atherosclerosis, stroke, arteriovenous malformations, and neurodegenerative diseases. Yet, the molecular mechanisms involved in high flow response are still poorly understood. Here, we described the development and validation of a 96-wells fluidic system, with interchangeable cell culture and fluidics, to perform high-throughput screenings under laminar high-flow conditions. We demonstrated that endothelial cells in our newly developed 96-wells fluidic system respond to fluid flow-induced shear stress by aligning alon

SUBMITTER: Fonseca CG 

PROVIDER: S-EPMC10504069 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature