A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor.

ABSTRACT: Vectors that facilitate the engineering of T cells that can better harness endogenous immunity and overcome suppressive barriers in the tumour microenvironment would help improve the safety and efficacy of T-cell therapies for more patients. Here we report the design, production and applicability, in T-cell engineering, of a lentiviral vector leveraging an antisense configuration and comprising a promoter driving the constitutive expression of a tumour-directed receptor and a second promoter enabling the efficient activation-inducible expression of a genetic payload. The vector allows for the delivery of a variety of genes to human T cells, as we show for interleukin-2 and a microRNA-based short hairpin RNA for the knockdown of the gene coding for haematopoietic progenitor kinase 1, a nega

SUBMITTER: Reichenbach P 

PROVIDER: S-EPMC10504085 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature