Renal tubular function and morphology revealed in kidney without labeling using three-dimensional dynamic optical coherence tomography.

ABSTRACT: Renal tubule has distinct metabolic features and functional activity that may be altered during kidney disease. In this paper, we present label-free functional activity imaging of renal tubule in normal and obstructed mouse kidney models using three-dimensional (3D) dynamic optical coherence tomography (OCT) ex vivo. To create an obstructed kidney model, we ligated the ureter of the left kidney for either 7 or 14 days. Two different dynamic OCT (DOCT) methods were implemented to access the slow and fast activity of the renal tubules: a logarithmic intensity variance (LIV) method and a complex-correlation-based method. Three-dimensional DOCT data were acquired with a 1.3 [Formula: see text]m swept-source OCT system and repeating raster scan protocols. In the normal kidney, the renal tubule

SUBMITTER: Mukherjee P 

PROVIDER: S-EPMC10504276 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature