Paired ATAC- and RNA-seq offer insight into the impact of HIV on alveolar macrophages: a pilot study.

ABSTRACT: People with HIV remain at greater risk for both infectious and non-infectious pulmonary diseases even after antiretroviral therapy initiation and CD4 cell count recovery. These clinical risks reflect persistent HIV-mediated defects in innate and adaptive immunity, including in the alveolar macrophage, a key innate immune effector in the lungs. In this proof-of-concept pilot study, we leveraged paired RNA-seq and ATAC-seq analyses of human alveolar macrophages obtained with research bronchoscopy from people with and without HIV to highlight the potential for recent methodologic advances to generate novel hypotheses about biological pathways that may contribute to impaired pulmonary immune function in people with HIV. In addition to 35 genes that were differentially expressed in macrophages

SUBMITTER: Staitieh BS 

PROVIDER: S-EPMC10504379 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature