SIRT1 activation promotes energy homeostasis and reprograms liver cancer metabolism.

ABSTRACT:

Background

Cancer cells are characterized by uncontrolled cell proliferation and impaired bioenergetics. Sirtuins are a family of highly conserved enzymes that play a fundamental role in energy metabolism regulation. SIRT1, in particular, drives many physiological stress responses and metabolic pathways following nutrient deprivation. We previously showed that SIRT1 activation using SCIC2.1 was able to attenuate genotoxic response and senescence. Here, we report that in hepatocellular carcinoma (HCC) cells under glucose-deprived conditions, SCIC2.1 treatment induced overexpression of SIRT1, SIRT3, and SIRT6, modulating metabolic response.

Methods

Flow cytometry was used to analyze the cell cycle. The MTT assay and xCELLigence system were used to measure cell viability and pr

SUBMITTER: Varghese B 

PROVIDER: S-EPMC10504761 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature