ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling.

ABSTRACT:

Background

Hepatocellular Carcinoma (HCC) possesses the high mortality in cancers worldwide. Nevertheless, the concrete mechanism underlying HCC proliferation remains obscure. In this study, we show that high expression of ARF6 is associated with a poor clinical prognosis, which could boost the proliferation of HCC.

Methods

Immunohistochemistry and western blotting were used to detect the expression level of ARF6 in HCC tissues. We analyzed the clinical significance of ARF6 in primary HCC patients. We estimated the effect of ARF6 on tumor proliferation with in vitro CCK8, colony formation assay, and in vivo nude mouse xenograft models. Immunofluorescence was conducted to investigate the ARF6 localization. western blotting was used to detect the cell cycle-related proteins wi

SUBMITTER: Hu Y 

PROVIDER: S-EPMC10505330 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature