Dataset Information

The GPx-1 Gene Variants (rs1050450) in Obesity: Association with the Risk of Obesity and the GPx Activity in Females.

ABSTRACT:

Background

This study aimed to investigate the GPx-1 gene polymorphism (rs1050450), the level of oxidative stress and antioxidant parameters, and the lipid profile in an obese Kurdish population in Sulaimani, Iraq.

Methods

In a case-control study,134 obese subjects and 131 normal BMI healthy individuals participated. The GPx-1 gene polymorphism was assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The levels of biochemical and oxidative parameters were determined using photometric methods.

Results

The results showed that the fasting blood sugar (FBS), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher in obese subjects compared to the control group. Obese individuals had significantly lower levels of high-density lipoprotein cholesterol (HDL-C) than the controls. The GPx-1 activity and total antioxidant capacity (TAC) levels were significantly elevated in the obese group compared to the control group (P=0.006, and P<0.001, respectively). No significant difference was detected in genotype and allele frequencies of GPx-1 (rs1050450) between obese and normal BMI groups. However, the presence of the GPx-1 TT genotype enhanced the risk of obesity in females by 1.93-fold (95% CI 1.04-3.58, P=0.036). In the total population, the GPx activity increased in the presence of TT compared to CC+CT and CT genotypes.

Conclusion

The study indicated that obesity is linked to significantly higher levels of FBS, TG, LDL-C, TAC, and GPx activity and lower level of HDL-C. Also, we found the GPx-1 gene polymorphism was associated with the risk of obesity in females and increased the GPx activity.

SUBMITTER: Ahmad AA

PROVIDER: S-EPMC10505473 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Publications

The <i>GPx-1</i> Gene Variants (rs1050450) in Obesity: Association with the Risk of Obesity and the GPx Activity in Females.

Ahmad Avan Arif AA Rahimi Zohreh Z Asadi Soheila S Vaisi-Raygani Asad A Kohsari Maryam M

Reports of biochemistry & molecular biology 20230401 1

<h4>Background</h4>This study aimed to investigate the GP<i>x-1</i> gene polymorphism (rs1050450), the level of oxidative stress and antioxidant parameters, and the lipid profile in an obese Kurdish population in Sulaimani, Iraq.<h4>Methods</h4>In a case-control study,134 obese subjects and 131 normal BMI healthy individuals participated. The GP<i>x-1</i> gene polymorphism was assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The levels of biochemi ...[more]

PMID: 37724151

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