Circadian disruption decreases gluconeogenic flux in late-gestation, nonlactating dairy cows.

ABSTRACT: Cattle exposed to shifts in light-dark phases during late pregnancy develop hypoglycemia and insulin resistance. Our objective was to investigate if differences in liver carbon flux for gluconeogenesis were driving circadian-disrupted metabolic alterations in glucose homeostasis, and relate changes in carbon flux to hepatic gene expression. We hypothesized circadian disruption would decrease hepatic carbon flux for glucose synthesis. Milking was ceased in late-gestation Holstein cows (n = 8) at 60 d before expected calving (BEC), and animals were assigned to either a control (n = 4) or a phase-shifted (PS; n = 4) group. From d 35 to 21 BEC both groups of cows were exposed to 16 h of light and 8 h of dark, but for the PS, light was shifted forward 6 h every 3 d. On d 21 BEC, liver biopsies

SUBMITTER: Beckett LM 

PROVIDER: S-EPMC10505771 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature