Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells.

ABSTRACT: The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein interactions take place. Here, we outline a protocol for the spatial interrogation of the centrosome proteome in human cells, such as those differentiated from induced pluripotent stem cells (iPSCs), through co-immunoprecipitation of protein complexes around selected baits that are known to reside at different structural parts of the centrosome, followed by mass spectrometry. The protocol describes expansion and differentiation of human iPSCs to dorsal forebrain neural progenitors and cortical projection neurons, harvesting and lysis of cells for protein

SUBMITTER: Uzbas F 

PROVIDER: S-EPMC10505934 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature