Single-cell multiomics of human fetal hematopoiesis define a developmental-specific population and a fetal signature.

ABSTRACT: Knowledge of human fetal blood development and how it differs from adult blood is highly relevant to our understanding of congenital blood and immune disorders and childhood leukemia, of which the latter can originate in utero. Blood formation occurs in waves that overlap in time and space, adding to heterogeneity, which necessitates single-cell approaches. Here, a combined single-cell immunophenotypic and transcriptional map of first trimester primitive blood development is presented. Using CITE-seq (cellular indexing of transcriptomes and epitopes by sequencing), the molecular profile of established immunophenotype-gated progenitors was analyzed in the fetal liver (FL). Classical markers for hematopoietic stem cells (HSCs), such as CD90 and CD49F, were largely preserved, whereas CD135 (F

SUBMITTER: Sommarin MNE 

PROVIDER: S-EPMC10506049 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature