Nitric oxide-dependent immunosuppressive function of thymus-derived mesenchymal stromal/stem cells.

ABSTRACT:

Background

The thymus is required for T cell development and the formation of the adaptive immunity. Stromal cells, which include thymic epithelial cells (TECs) and mesenchymal stromal cells (MSCs), are essential for thymic function. However, the immunomodulatory function of thymus-derived MSCs (T-MSCs) has not been fully explored.

Methods

MSCs were isolated from mouse thymus and their general characteristics including surface markers and multi-differentiation potential were characterized. The immunomodulatory function of T-MSCs stimulated by IFN-γ and TNF-α was evaluated in vitro and in vivo. Furthermore, the spatial distribution of MSCs in the thymus was interrogated by using tdTomato-flox mice corssed to various MSC lineage Cre recombinase lines.

Results

A subset o

SUBMITTER: Su X 

PROVIDER: S-EPMC10506207 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature