TLR8 escapes X chromosome inactivation in human monocytes and CD4⁺ T cells.

ABSTRACT:

Background

Human endosomal Toll-like receptors TLR7 and TLR8 recognize self and non-self RNA ligands, and are important mediators of innate immunity and autoimmune pathogenesis. TLR7 and TLR8 are, respectively, encoded by adjacent X-linked genes. We previously established that TLR7 evades X chromosome inactivation (XCI) in female immune cells. Whether TLR8 also evades XCI, however, has not yet been explored.

Method

In the current study, we used RNA fluorescence in situ hybridization (RNA FISH) to directly visualize, on a single-cell basis, primary transcripts of TLR7 and TLR8 relative to X chromosome territories in CD14⁺ monocytes and CD4⁺ T lymphocytes from women, Klinefelter syndrome (KS) men, and euploid men. To assign X chromosome territories in cel

SUBMITTER: Youness A 

PROVIDER: S-EPMC10506212 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature