Analysis of a macrophage carbamylated proteome reveals a function in post-translational modification crosstalk.

ABSTRACT:

Background

Lysine carbamylation is a biomarker of rheumatoid arthritis and kidney diseases. However, its cellular function is understudied due to the lack of tools for systematic analysis of this post-translational modification (PTM).

Methods

We adapted a method to analyze carbamylated peptides by co-affinity purification with acetylated peptides based on the cross-reactivity of anti-acetyllysine antibodies. We also performed immobilized-metal affinity chromatography to enrich for phosphopeptides, which allowed us to obtain multi-PTM information from the same samples.

Results

By testing the pipeline with RAW 264.7 macrophages treated with bacterial lipopolysaccharide, 7,299, 8,923 and 47,637 acetylated, carbamylated, and phosphorylated peptides were identified, respec

SUBMITTER: You Y 

PROVIDER: S-EPMC10506243 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature