Immobilised-enzyme microreactors for the identification and synthesis of conjugated drug metabolites.

ABSTRACT: The study of naturally circulating drug metabolites has been a focus of interest, since these metabolites may have different therapeutic and toxicological effects compared to the parent drug. The synthesis of metabolites outside of the human body is vital in order to conduct studies into the pharmacological activities of drugs and bioactive compounds. Current synthesis methods require significant purification and separation efforts or do not provide sufficient quantities for use in pharmacology experiments. Thus, there is a need for simple methods yielding high conversions whilst bypassing the requirement for a separation. Here we have developed and optimised flow chemistry methods in glass microfluidic reactors utilising surface-immobilised enzymes for sulfonation (SULT1a1) and glucuronid

SUBMITTER: Doyle B 

PROVIDER: S-EPMC10506384 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature