Cyanine Masking: A Strategy to Test Functional Group Effects on Antibody Conjugate Targeting.

ABSTRACT: Conjugates of small molecules and antibodies are broadly employed diagnostic and therapeutic agents. Appending a small molecule to an antibody often significantly impacts the properties of the resulting conjugate. Here, we detail a systematic study investigating the effect of various functional groups on the properties of antibody-fluorophore conjugates. This was done through the preparation and analysis of a series of masked heptamethine cyanines (CyMasks)-bearing amides with varied functional groups. These were designed to exhibit a broad range of physical properties, and include hydrophobic (-NMe₂), pegylated (NH-PEG-8 or NH-PEG-24), cationic (NH-(CH₂)₂NMe₃⁺), anionic (NH-(CH₂)₂SO₃^-), and

SUBMITTER: Thapaliya ER 

PROVIDER: S-EPMC10506421 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature