Spatial transcriptomics delineates molecular features and cellular plasticity in lung adenocarcinoma progression.

ABSTRACT: Indolent (lepidic) and aggressive (micropapillary, solid, and poorly differentiated acinar) histologic subtypes often coexist within a tumor tissue of lung adenocarcinoma (LUAD), but the molecular features associated with different subtypes and their transitions remain elusive. Here, we combine spatial transcriptomics and multiplex immunohistochemistry to elucidate molecular characteristics and cellular plasticity of distinct histologic subtypes of LUAD. We delineate transcriptional reprogramming and dynamic cell signaling that determine subtype progression, especially hypoxia-induced regulatory network. Different histologic subtypes exhibit heterogeneity in dedifferentiation states. Additionally, our results show that macrophages are the most abundant cell type in LUAD, and identify diffe

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10507052 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature