Whole-genome screens reveal regulators of differentiation state and context-dependent migration in human neutrophils.

ABSTRACT: Neutrophils are the most abundant leukocyte in humans and provide a critical early line of defense as part of our innate immune system. We perform a comprehensive, genome-wide assessment of the molecular factors critical to proliferation, differentiation, and cell migration in a neutrophil-like cell line. Through the development of multiple migration screen strategies, we specifically probe directed (chemotaxis), undirected (chemokinesis), and 3D amoeboid cell migration in these fast-moving cells. We identify a role for mTORC1 signaling in cell differentiation, which influences neutrophil abundance, survival, and migratory behavior. Across our individual migration screens, we identify genes involved in adhesion-dependent and adhesion-independent cell migration, protein trafficking, and reg

SUBMITTER: Belliveau NM 

PROVIDER: S-EPMC10507112 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature