Multi-omics approach reveals dysregulated genes during hESCs neuronal differentiation exposure to paracetamol.

ABSTRACT: Prenatal paracetamol exposure has been associated with neurodevelopmental outcomes in childhood. Pharmacoepigenetic studies show differences in cord blood DNA methylation between unexposed and paracetamol-exposed neonates, however, causality and impact of long-term prenatal paracetamol exposure on brain development remain unclear. Using a multi-omics approach, we investigated the effects of paracetamol on an in vitro model of early human neurodevelopment. We exposed human embryonic stem cells undergoing neuronal differentiation with paracetamol concentrations corresponding to maternal therapeutic doses. Single-cell RNA-seq and ATAC-seq integration identified paracetamol-induced chromatin opening changes linked to gene expression. Differentially methylated and/or expressed genes were

SUBMITTER: Spildrejorde M 

PROVIDER: S-EPMC10507163 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature