Engineering a CEACAM1 Variant with the Increased Binding Affinity to TIM-3 Receptor

ABSTRACT:

Background

T-cell immunoglobulin domain and mucin domain-3 (TIM-3) is an inhibitory receptor expressed in a variety of cells, including dendritic cells, T-helper 1 lymphocytes, and natural killer cells. Binding of this protein to its ligand, carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), causes T-cell exhaustion, a specific condition in which effector T cells lose their ability to proliferate and produce cytokines. Blocking this inhibitory receptor is known to be an effective strategy for treating cancer and other related diseases. Therefore, in this study, in order to block the inhibitory receptor of TIM-3, we designed and produced recombinantly a protein with a high binding affinity to this receptor.

Methods

The extracellular domain of CEACAM1 involve

SUBMITTER: Hajihassan Z 

PROVIDER: S-EPMC10507288 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature