Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.

ABSTRACT: In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress to achieve high product concentrations. Macroparticles were added to shake flask cultures of the filamentous actinomycete Lentzea aerocolonigenes to find these optimal cultivation conditions. However, there is currently no model concept for the dependence of the strength and frequency of the bead-induced stress on the process parameters. Therefore, shake flask simulations were performed for combinations of bead size, bead concentration, bead density and shaking frequency. Contact analysis showed that the highest shear stresses were c

SUBMITTER: Schrader M 

PROVIDER: S-EPMC10507416 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature