Cell encapsulation in gelatin methacryloyl bioinks impairs microscale diffusion properties.

ABSTRACT: Light-assisted bioprinted gelatin methacryloyl (GelMA) constructs have been used for cell-laden microtissues and organoids. GelMA can be loaded by desired cells, which can regulate the biophysical properties of bioprinted constructs. We study how the degree of methacrylation (MA degree), GelMA mass concentration, and cell density change mass transport properties. We introduce a fluorescent-microscopy-based method of biotransport testing with improved sensitivity compared to the traditional particle tracking methods. The diffusion capacity of GelMA with a higher MA significantly decreased compared to a lower MA. Opposed to a steady range of linear elastic moduli, the diffusion coefficient in GelMA varied when cell densities ranged from 0 to 10 × 10⁶ cells/ml. A comparative study

SUBMITTER: Dogan E 

PROVIDER: S-EPMC10507472 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature