A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus.

ABSTRACT:

Background

Enzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic expression hosts such as Thermus thermophilus [T. thermophilus] can overcome specific challenges experienced with protein production in mesophilic expression hosts, such as leading to better folding, increased protein stability, solubility, and enzymatic activity. However, available inducible promoters for efficient protein production in T. thermophilus HB27 are limited.

Results

In this study, we characterized the pilA4 promoter region and evaluated its potential as a tool for production of thermostable enzymes in T. thermophilus HB27. Reporter gene analysis using a promoterless β-glucosidase gene revealed that the pilA4 promoter is h

SUBMITTER: Kirchner L 

PROVIDER: S-EPMC10507856 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature