Developing diagnostic tools for canine periodontitis: combining molecular techniques and machine learning models.

ABSTRACT:

Background

Dental plaque microbes play a key role in the development of periodontal disease. Numerous high-throughput sequencing studies have generated understanding of the bacterial species associated with both canine periodontal health and disease. Opportunities therefore exist to utilise these bacterial biomarkers to improve disease diagnosis in conscious-based veterinary oral health checks. Here, we demonstrate that molecular techniques, specifically quantitative polymerase chain reaction (qPCR) can be utilised for the detection of microbial biomarkers associated with canine periodontal health and disease.

Results

Over 40 qPCR assays targeting single microbial species associated with canine periodontal health, gingivitis and early periodontitis were developed and validat

SUBMITTER: Ruparell A 

PROVIDER: S-EPMC10507867 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature