A fluorescence-based assay for Trichomonas vaginalis drug screening.

ABSTRACT:

Background

The emergence and spread of drug resistance in Trichomonas vaginalis parasites has become an important concern in trichomoniasis treatment. Fast and reliable growth assessment is critical for validating in vitro drug susceptibility and high-throughput screening of newly developed drugs.

Methods

Modified media without yeast extract were evaluated for their ability to support the growth of T. vaginalis parasites. The potential of the nucleic acid-binding dye SYBR Green I for detecting T. vaginalis drug resistance was characterized, and seeding parasite concentration and incubation time were optimized. The fluorescence assay based on SYBR Green I was further validated in four T. vaginalis isolates with different susceptibilities to the antibiotics metronidazole, tini

SUBMITTER: Chen Q 

PROVIDER: S-EPMC10507874 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature