Wnt and BMP signalling direct anterior-posterior differentiation in aggregates of mouse embryonic stem cells.

ABSTRACT: Stem-cell-based embryo models have allowed greater insight into peri-implantation mammalian developmental events that are otherwise difficult to manipulate due to the inaccessibility of the early embryo. The rapid development of this field has resulted in the precise roles of frequently used supplements such as N2, B27 and Chiron in driving stem cell lineage commitment not being clearly defined. Here, we investigate the effects of these supplements on embryoid bodies to better understand their roles in stem cell differentiation. We show that Wnt signalling has a general posteriorising effect on stem cell aggregates and directs differentiation towards the mesoderm, as confirmed through the upregulation of posterior and mesodermal markers. N2 and B27 can mitigate these effects and upregulate

SUBMITTER: Amel A 

PROVIDER: S-EPMC10508691 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature