The Egg-Counter: A novel microfluidic platform for characterization of Caenorhabditis elegans egg-laying.

ABSTRACT: Reproduction is a fundamental process that shapes the demography of every living organism yet is often difficult to assess with high precision in animals that produce large numbers of offspring. Here, we present a novel microfluidic research platform for studying Caenorhabditis elegans' egg-laying. The platform provides higher throughput than traditional solid-media assays while providing a very high degree of temporal resolution. Additionally, the environmental control enabled by microfluidic animal husbandry allows for experimental perturbations difficult to achieve with solid-media assays. We demonstrate the platform's utility by characterizing C. elegans egg-laying behavior at two commonly used temperatures, 15 and 20°C. As expected, we observed a delayed onset of egg-lay

SUBMITTER: Banse SA 

PROVIDER: S-EPMC10508723 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature